By combining traditional plant breeding with ever-faster genetic sequencing tools, researchers are making fruits and vegetables more flavorful, colorful, shapely and nutritious
Armed with toothpicks and sour cream, Michael Mazourek and three friends marched into the field of 600 chili pepper plants. One by one, they pierced the habaneros and brought the juices to their tongues, cooling and cleansing their palates with cream now and then. Habaneros are typically some of the hottest chilies around, ranking as high as 350,000 on the Scoville scale of pepper pungency—40 times hotter than the jalapeno. The habaneros Mazourek and his team had grown in this experimental field at Cornell University were in no way typical, however. The four graduate students knew that, due to an unidentified genetic mutation, a portion of the peppers would not be very spicy. Exactly what they would taste like was a mystery.
If a particular habanero’s juices indicated that it was relatively mild, they bit into it like an apple. Some of the peppers were smoky; others astringent. Some tasted faintly of sausage; others had the foul funk of gym socks. But the most interesting peppers by far possessed all the complex aroma and flavor of a habanero, yet none of its heat.
A few months earlier Mazourek had received a shipment of seeds from researchers at New Mexico State University. These seeds, they said, grew into habanero plants whose red peppers did not even register on the Scoville scale. Knowing that Mazourek was a plant breeder interested in pepper pungency, the New Mexico scientists thought he might like to study the tame habaneros himself.
Mazourek raised the seeds he got in the mail into adult plants and mated them with ordinary habaneros. Inside greenhouses he carefully transferred pollen between flowers on the two kinds of plants with tweezers, ensuring that the resulting offspring and, eventually, the 600 grandchildren would have a mix of their parents’ DNA. After growing that third generation in a quarter-acre field, he recruited some fellow scholars for the intrepid taste test.
As the friends ate the unusual peppers, they snipped off leaves from each plant, which they later crushed and liquefied to examine the DNA within. By syncing the results of the DNA analysis with what their palates had told them, the team managed to identify a specific genetic mutation that explained why some of the peppers lacked heat. Chili peppers get their fire from an oily molecule called capsaicin, which is produced by glands within the fruit—those leathery, seed-covered white or yellow bits that people usually cut out of a pepper. As it turns out, the same bucket brigade of chemical reactions responsible for making capsaicin is also essential for concocting a bouquet of associated compounds that give a pepper its scent. The heatless habaneros had inherited a mutation that nearly—but not completely—shut down their capsaicin factories, leaving the glands just functional enough to eke out a drop of capsaicin and fragrant molecules here and there.
Seeing a unique opportunity, Mazourek and his colleagues spent the next several years making crosses between various plants in an attempt to create a single variety that reliably produced habaneros with plenty of zest but no heat. Having illuminated pungency genetics, they no longer had to wait for plants to grow up and work their taste buds overtime simply to find out if the peppers were spicy or not. Instead, they could take a leaf from a young plant, analyze its DNA and determine whether it would produce blazing or bland peppers before deciding whether to raise it to maturity—a trick that made the whole breeding process more efficient. Today, their prized pepper is persimmon orange, a bit longer than a typical habanero and wonderfully piquant. They call it a habanada, as in no burning heat—nada, zip. Cornell hopes to license the habanada to a major seed company soon for wider distribution…
READ FULL ARTICLE HERE… | Scientific American
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